小鼠胸段脊髓背横切后Lingo-1的表达变化

The expression of Lingo-1 after thoracic spinal cord dorsal laceration in mice

目的观察Lingo-1在小鼠脊髓背侧切割伤后不同时间窗的动态表达变化。方法采用LISA脊髓损伤仪制作小鼠第9胸髓背侧切割伤模型(深度1.1 mm),运用Real time RT-PCR和Western blot观察Lingo-1 mRNA和蛋白在术后1、3、7、14、28 d的表达变化。结果 Lingo-1 mRNA和蛋白的表达在小鼠脊髓背侧切割伤后1 d即显著上调,7 d时达高峰,并持续上调表达至术后2～4周。结论 Lingo-1在脊髓切割伤后表达上调,揭示其与脊髓损伤后轴突变性有关;对于治疗脊髓损伤促进轴突再生,Lingo-1拮抗剂应在损伤早期即开始并持续使用。

Objective To explore the dynamic expression of Lingo-1 after spinal cord dorsal laceration in the mice. Methods LISA （Louisville Injury Systems Apparatus） was used to generate a precise 9th thoracic dorsal laceration with the lesion depth of 1. lmm in the mice. Real time RT-PCR and Western Blot were applied to access the expression levels of Lingo-1 mRNA and protein at 1, 3, 7, 14, and 28 days post-injury. Results The expression levels of Lingo-1 mRNA and protein were significantly up- regulated at 1 day and reached the peak value at 7 days post-surgery. The up-regulation of Lingo-1 mRNA lasted to the 14 days and the up-regulation of Lingo-1 protein lasted to the 28 days post-surgery compared to the normal controls. Conclusion These results showed the higher expression of Lingo-1 after dorsal spinal cord laceration and suggested which was involved in the axonal degenera- tion after spinal cord injury （SCI）. The early and sustained usage of Lingo-1 antagonist was suggested to promote the axonal regenel ation after SCI.