酶联免疫法测定牡蛎中腹泻性毒素的研究

The study on detection of Diarrhetic shellfish poisons by ELISA in Ostrea rivularis Gould

运用酶联免疫法对海洲湾海域赤潮监控区、增养殖区、趋势性监测区的养殖牡蛎进行腹泻性毒素监测。连续3年的监测结果显示:海洲湾贝类监控区牡蛎的腹泻性毒素检出率较高,达71.4%,每年呈逐年下降趋势,降幅达25.3%。在3个监测区域腹泻性毒素检出率分别为83.3%、100%、66.7%,以赤潮监控区牡蛎腹泻性毒素检出率最高。在样品中添加1.0、3.0和5.0μg/kg腹泻性毒素标准品,平均回收率分别达到88.0%,86.7%和88.6%。批内变异系数1.44%～4.60%,批间变异系数3.08%～3.98%。酶联免疫法重复性较好,适用于海产贝类中腹泻性毒素的检测。

Detections of diarrhetic shellfish poisons in Ostrea rivularis Gould were accomplished by ELISA method from the red tide-monitoring area,culture coastal area and tendency-monitoring area of Haizhou Bay in recent 3 years,the results indicated that the detectable rate of diarrhetic shellfish poisons in Ostrea rivularis Gould from the red tide-monitoring area was high and reached 71.4%,it was decreased year by year in recent 3 years with 25.3% decreasing amplitude. Of the 3 monitoring areas,the detectable rate of DSP in Ostrea rivularis Gould samples of red tide-monitoring area was the highest. The average recoveries were 88.0%,86.7%,88.6% respectively when the samples were spiked with 1.0 μg/kg,3.0 μg/kg,5.0 μg/kg of DSP. The relative standard deviations（n=3） for intra assay are 1.44%-4.60% and coefficients of variation for inter-assay were 3.08%-3.98%. The method has good reproducibility,can be used to detect content of DSP in Ostrea rivularis Gouht.