饲料着色剂加丽素红对鸡血清蛋白的影响

Effects of Carophyll Red on Content of Serum Proteins in Chickens

研究加丽素红对鸡血清蛋白的影响,为科学评价加丽素红的使用安全性提供依据。根据体重对等原则,将120只健康的19周龄海兰蛋鸡随机分为对照组(0g/kg)、低剂量组(0.08g/kg)和高剂量组(8g/kg)3组。首先饲喂添加不同剂量加丽素红的试验饲料30d,之后改喂基础饲料至60d,分别在10、20、30、40、50、60、70、90d于颈静脉采血,采用SDS-PAGE分离鸡血清蛋白,并利用凝胶成像系统进行扫描定量。结果表明,饲喂添加加丽素红饲料的海兰蛋鸡血清中的4种血清蛋白α球蛋白、β球蛋白、γ球蛋白和血清白蛋白随饲喂天数的增加,不同剂量产生不同程度的差异变化,加丽素红对鸡血清蛋白的合成有影响。

To provide scientific data for the security use of carophyll red, the effects of carophyll red on con- tent of chicken serum proteins were studied in this research. According to the principle of equal weight, one hundred and twenty Hyline layers at nineteen weeks were randomly divided into control group, low dose and high dose group feeding with carophyll red at 0 g/kg, 0.08 g/kg and 8 g/kg respectively. Chick- ens were fed with different dose of carophyll red added feed for 30 days and fed for 60 days with basal feed after that. At the feeding time of 10th, 20th, 30th, 40th, 50~h, 60th, 70~h and 90~h day, the blood samples were collected through jugular vein and sera were isolated, contents of serum proteins were separated by SDS-PAGE and scanned by using gel imaging-forming system. Research results found that contents of four serum proteins （a globulin, 13 globulin, 9, globulin, and serum albumin in Hyline chicken were increase with the feed time, different doses produced different degrees of difference in change. The use of earophyll red could affect protein synthesis in chickens.