家兔PRK术后角膜组织病理学的实验研究

A HISTOPATHOLOGIC STUDY OF WOUND HEALING IN RABBIT CORNEA AFTER EXCIMER LASER PHOTOREFRACTIVE KEPATECTOMY

目的 评价实验性家兔不同屈光度PRK术后角膜创面愈合的过程。方法 应用SVSAPEXPLUS型准分子激光治疗系统对 6只家兔双眼进行PRK术。结果 术后 3d ,角膜上皮细胞 1层或 2层覆盖切削区。术后 30d ,可见角膜上皮细胞增生 ,体积增大 ,细胞由正常的 5层或 6层增加到 7层或 8层。术后10 0d ,角膜上皮细胞基本恢复正常。结论 PRK术后 10 0d角膜组织结构基本正常 ,但仍有组织薄弱处和特殊性改变。

Objective To evaluate the corneal healing of non-equality diopter response histopathology after excimer laser photorefractive keratectomy(PRK) with SVS APEX PLUS excimer laser. Methods PRK was performed on both eyes of 6 white rabbits (12 eyes). Results At the end of 3 days postoperatively one or two layers of corneal epithelial cells covered the ablation zone. Beneach the ablation zone, the corneal collagen fibers appeared in abnormal aligment and showed any damage. At the end of 30 days,the epithelial cells showed hyperplastic changes.The cells were larger and increased to 7 or 8 layers from the nomal 5 or 6 layers.100 days after surgery. the corneal epithelium returned to mormal again.Conclusion Despite recovery of a continuous and smooth epithelial layer and near nomal corneal tissues 100 days after PPK,abnrmalities of both epithelial and superficial stroma could be detected in the area of ablation.