两种成骨细胞对硝苯地平转运的对比研究

Biological transport of nifedipine by two kinds of osteoblasts

目的研究成骨细胞对硝苯地平的跨膜转运,为种植体全身给药提供实验依据。方法将MC3T3-E1细胞和大鼠下颌骨成骨细胞(mandibular osteoblasts,MO)分别与含有硝苯地平的磷酸盐缓冲液(PBS)共同孵育一定时间后,收集细胞,超声波破碎,用高效液相色谱法测定裂解液中的药物含量,考马斯亮蓝法测定细胞蛋白总量。将细胞内药物含量与细胞蛋白的比值作为观察药物转运量的检测指标。结果两种成骨细胞内均可测到硝苯地平的存在,转运药物的量与药物的浓度及药物的孵育时间有关。结论成骨细胞可以跨膜转运硝苯地平进入细胞内,药物的转运量与药物浓度和孵育时间相关。

Objective It is to investigate transmembrane transport of nifedipine by osteoblasts and verify the feasibility transmembrane transport of nifedipine by osteoblasts, verifying the feasibility of local drug delivery in the design of dental implant administration system. Methods Incubated the MC3T3-E1 cells and mandibular osteoblasts（MO） with agent solutions. After incubation for indicated times （ 1, 5, 10, 15, 20, 30, 45 min） , the agent solutions were quickly removed. The cells were collected and were disrupted by ultrasonic wave. The lysate agent concentration was measured by high performance liquid chromatography （HPLC） and the total proteins were measured by Bradford assay. Results Nifedipine could be transported by both kinds of cells. The intracellular contents were dependent on the extracellular concentration and incubation time. Conclusions Osteoblasts can transport nifedipine into cells.