甘氨酸对大鼠前额叶皮质锥体神经元串连动作电位影响的研究

Effects of Glycine on Trains of Action Potentials in Pyramidal Neurone of Rat Prefrontal Cortex

目的:研究甘氨酸对大鼠前额叶皮质锥体神经元串连动作电位的影响。方法:大鼠前额叶皮质切片,全细胞膜片钳记录锥体神经元串连动作电位。结果:甘氨酸(300μmol/L)可显著降低大鼠前额叶皮质锥体神经元串连动作电位的发放频率,其作用可被番木鳖碱(1μmol/L)所阻断,表明其作用部位为番木鳖碱依赖性甘氨酸受体。甘氨酸还引起膜超极化,延长动作电位的潜伏期,加快复极化进程。结论:前额叶皮质的甘氨酸受体可能在VTA-NAcc-PFC神经环路的调控中发挥重要作用。

Objective： To study the effect of glycine on trains of action potentials in pyramidal neurone of rat prefrontal cortex. Methods ： Rat prefrontal cortex slices were prepared. Trains of action potentials were investigated using whole-cell patch-clamp recording in pyramidal neurone. Results ： The frequency of trains of action potentials in pyramidal neurone was decreased significantly by 300 μmol/L glycine, this effect could be blocked by 1 μmol/L strychnine, demonstrating that it acts on strychnine-dependent glycine receptors. The glycine can cause membrane hyperpolariza- tion, prolong the latent period of action potential, accelerate the repolarization process. Conclusion ： The glycine receptors in prefrontal cortex may involve in important function in regulation of VTA-NAcc-PFC neural circuit.