不同光照条件对黄瓜幼苗的生理响应

Physiological Response of Cucumber Seedlings Under Different Light Intensity

探明不同光照条件对黄瓜幼苗的生理响应。本试验以"津春4号"黄瓜品种为材料,在黄瓜幼苗长至两叶一心时,分别置于不同光照下生长6 d,然后取第2片真叶测定相关指标。结果表明,在弱光下黄瓜叶片丙二醛(MDA)含量、超氧阴离子(O2.-)生成速率以及过氧化氢(H2O2)含量均有显著提高,过氧化氢酶(CAT)活力有所降低,抗坏血酸过氧化物酶(APX)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPX)和谷胱甘肽还原酶(GR)活性有显著提高。

In order to investigate the physiological response of cucumber seedlings, seedlings of Cucumber Jinchun No. 4 were cultivated at different light intensities for 6 days when the second leaves were fully expanded. Samples of the second leaves were used to determine the activities of antioxidants such as ascorbate peroxidase （APX）, superoxide dismutase （SOD）, catalase （CAT）, guaiacol peroxidase （GPX）, glutathione reductase （GR） and assay the cntents of malondialdehyde （MDA）, H2O2 and superoxide anion formation rate （O2.）. The results revealed that low light significantly increased antioxidant activities of cucumber leaves such as APX, SOD, GPX and GR, cntents of MDA, H2O2 and superoxide anion formation rate and reduced the activity of CAT.