胃癌患者外周血CD_(44)v_5含量的测定及临床意义

The Clinical Significance of The Contents of CD 44 v5 in Peripheral Blood of Patients With Gastric Cancer

目的 研究测定胃癌患者外周血淋巴细胞 (PBL)CD4 4变异体 5 (CD4 4v5)含量 ,探讨其对胃癌转移诊断的临床意义。方法 应用流式细胞术 (FCM )检测 30例胃癌患者术前PBLCD4 4v5含量和 10例正常对照组PBLCD4 4v5含量。结果 胃癌患者术前PBLCD4 4v5含量为 7 6 1%± 5 5 4% ,比正常对照组明显增高 (P <0 0 1)。有转移的胃癌患者PBLCD4 4v5含量 (8 77%± 5 95 % )明显高于无转移者 (4 44 %± 2 33% ) ,两者差异显著 (P <0 0 1)。结论 PBLCD4 4v5可望作为胃癌患者转移的生物学监测指标。应用FCM检测胃癌患者术前PBLCD4 4v5含量对胃癌转移的诊断和进展的评价具有重要的临床意义。

Objective To investigate the contents of CD 44 that shared exon variant 5(CD 44 v 5) in peripheral blood lymphocytes(PBL) of patients with gastric carcinoma and its clinical significance.Methods The contents of CD 44 v 5 were determined by means of flow cytometry in PBL of 30 patients with gastric carcinoma before surgery and 10 normal controls.Results The contents of CD 44 v 5 were significantly higher in PBL of patients with gastric carcinoma before surgery compared with those of controls( P <0 01).Node positive gastric cancer patients showed significantly elevated contents of CD 44 v 5 in PBL in comparison to node negative gastric cancer( P <0 01).Conclusion CD 44 v 5 in PBL may be useful as a matestatic marker.It may be of important clinical value in the diagnosis of metastasis and judgment of development for the patients with gastric cancer to analyse the contents of CD 44 v 5 in PBL by flow cytometry.