bFGF对慢性应激小鼠学习记忆及海马Ach含量的影响

Effects of bFGF on Learning-Memory Ability and the Content of Ach in Hippocampus in Chronic Stress Mice

目的探讨碱性成纤维细胞生长因子(Basic fibroblast growth factor,bFGF)对慢性应激模型小鼠学习记忆的影响及海马内Ach含量的变化.方法利用不确定应激方法建立慢性应激动物模型,共4周;应激第15天开始每日应激前腹腔注射bFGF,持续15 d;应用跳台法和避暗法观察bFGF对慢性应激小鼠学习记忆的作用;碱羟胺比色法检测乙酰胆碱(Ach)含量.结果 bFGF组小鼠学习记忆能力提高,海马Ach含量增高.结论 bFGF通过增加海马内胆碱能功能,改善慢性应激小鼠学习记忆能力.

Objective To investigate effects of basic fibroblast growth factor（bFGF）on the ability of learning and memory,and the content of Ach in hippocampus in chronic stress mice.Method An uncertain stress method was used for four weeks to establish the chronic stress model in mice.bFGF was injected intraperitoneally for 15 days on 15th day of the stress.The step-down test and darkness avoidance test were used to observe the effect of bFGF on learning-memory ability in the chronic stress mice.The change of Ach levels was tested with the hydroxylamine colorimetric assay.Results The results showed that the learning-memory abilities of mice in bFGF group were improved and contents of Ach in hippocampus increased.Conclusion bFGF can improve the learning-memory ability of chronic stress mice through improving the cholinergic function in hippocampus.