微孔板杂交法检测结核分枝杆菌的初步临床应用

Evaluation of the detection of mycobacterium tuberculosis in clinical specimens by PCR and a microtiter plate based DNA hybridization assay.

目的 :对 PCR-微孔板杂交法的临床应用进行评价。方法 :取临床标本 2 6 8份分别进行抗酸染色、培养、PCR扩增产物电泳、PCR微孔板杂交四种方法的比较。结果 :在 2 16份临床高度怀疑有结核分枝杆菌感染的患者标本中 ,PCR-微孔板杂交法检出阳性为 98例 ,其检出率高于 PCR-电泳法 (91/ 2 18)、培养法 (81/ 2 18)和抗酸染色法(5 6 / 2 18)。5 0份临床证实无结核分枝杆菌感染的病人标本 ,四种方法均未能检出结核分枝杆菌。结论 :PCR-微孔板杂交法是高度敏感。

To evaluate a novel microtiter plate based DNA hybridization assay (PCR plate)268 clinical samples were detected by PCR gel analysis、PCR microtiter plate hybridization assay、culture and Ziehl Neelsen stain respectively and the results were compared. Among the 218 clinical samples which may contain mycobacterium tuberculosis, 98 were positive by PCR plate, the sensitiviy was higher than PCR electrophoresis method(91/218), culture(81/218)and Ziehl Neelsen stain(56/218),and no positive was found by the four methods in 50 clinical samples without mycobacterium tuberculosis.Therefore PCR microtiter plate DNA hybridization assay can objectively detect mycobacterium tuberculosis from clinical specimens with high specificity and sensitivity.