发酵豆粕提取物中染料木素的分离纯化及结构鉴定

Purification and Identification of Genistein in Fermented Soybean Meal Extract

对发酵豆粕提取物经正丁醇处理后的甲醇可溶物,利用正相硅胶柱色谱及重结晶法从其提取物中分离出染料木素,经紫外光谱(UV)、质谱(MS)、核磁共振(NMR)等波谱学方法鉴定证实了其结构。高效液相色谱分析表明,所提纯的染料木素纯度达到了98%以上。

Fermented soybean meal extract was extracted by n-butanol,the raffinate phase was then extracted by methanol,and the methanol soluble material was concentrated by the vacuum evaporation of organic solvent.Genistein was isolated from the extract using silica gel column chromatography with a gradient elution of dichloromethane and methanol and further recrystallized in methanol.The structure of genistein was identified using ultraviolet spectroscopy（UV）,mass spectrometry（MS）,and nuclear magnetic resonance（1H NMR,13C NMR）.The HPLC chromatogram with UV at 260 nm showed that the purity of genistein was higher than 98%.