乙酸钠、水杨酸和Cu^(2+)对北柴胡不定根培养合成皂苷的影响

The effect of sodium acetate,salicylic acid and Cu^(2+) on biosynthesis of saikosaponins in adventitious root culture of Bupleurum chinense

通过添加柴胡皂苷等目的产物合成的外源前体物质和诱导子,探讨了北柴胡不定根培养高效合成柴胡皂苷的条件。结果表明,在1/2MS培养基中添加乙酸钠、水杨酸和Cu2+均有利于不定根合成柴胡皂苷。在培养基中添加0.8 g/L乙酸钠,有利于原产于山西万荣的北柴胡的不定根的皂苷合成;在培养基中添加0.6 g/L乙酸钠,有利于原产于山西左权的北柴胡的不定根的皂苷合成。在培养基中添加0.06 g/L水杨酸,有利于万荣、左权北柴胡不定根的皂苷合成。添加0.1 mg/L Cu2+,有利于原产于山西芮城的北柴胡的皂苷合成;添加0.25mg/L Cu2+,有利于万荣北柴胡不定根的皂苷合成。

The conditions of using Bupleurum chinense adventitious root system to produce saikosaponins efficient were studied by weans of adding precursors and elicitor.The results showed that the 1/2 MS medium which adding Sodium acetate,Salicylic acid and Cu2＋ had the effects on biotransform ation and biosynthesis of saikosaponins.The medium which adding 0.8 g/L sodium acetate helps to Biosynthesis of Saikosaponins in adventitious root system of Bupleurum chinense from Wanrong.The medium which adding 0.6 g/L sodium acetate helps to Biosynthesis of Saikosaponins in adventitious root system of Bupleurum chinense from Zuoquan.The medium which adding 0.06 g/L salicylic acid helps to Biosynthesis of Saikosaponins in adventitious root system of Bupleurum chinense from Wanrong and Zuoquan.The medium which adding 0.1 mg/L Cu2＋ helps to Biosynthesis of Saikosaponins in adventitious root system of Bupleurum chinense from Ruicheng.The medium which adding 0.25 mg/L Cu2＋ helps to Biosynthesis of Saikosaponins in adventitious root system of Bupleurum chinense from Wanrong.