前列腺癌组织中的端粒酶活性表达

Expression of Telomerase Activity in Prostate Cancer

目的 探讨前列腺癌组织中端粒酶活性表达与前列腺癌某些生物学行为的关系。方法 采用端粒重复序列扩增文件 -酶标法 ( TRAP- EL ISA )测定前列腺癌组织中端粒酶活性。结果 10例正常前列腺组织无端粒酶活性。 15例前列腺癌组织中 13例检出端粒酶活性 ,阳性率 86 %。端粒酶活性与肿瘤分化程度相关。 15例良性前列腺增生组织中 4例检出端粒酶活性 ,阳性率为 2 6 % ,其水平显著低于肿瘤组织。结论 端粒酶活性与前列腺癌的生物学行为有关 ,能估测前列腺癌的生物恶性潜力。

Objective To investigate the relationship between telomerase activity and the biological behavior of prostate cancer.Methods The telomerase activity in prostate cancer tissues was detected by TRAP( telomeric repeat amplification protocol) ELISA which is based upon amplification of the initial telomerase product and detected by ELISA.Results None of the 10 samples from normal prostates expressed telomerase activity. The thirteen of fifteen cases with prostate cancer presented telomerase acitivity. The activity of telomerase was associated with the pathological differentiation of prostate cancer. The four of fifteen cases with benign prostatic hyperplasia (BPH) expressed telomerase activity, and the activity of telomerase was lower than prostate cancer. Conclusions The telomerase activity may be related to biological behavior of prostate cancer and can evaluate the malignant potential of prostate cancer. The presence of telomerase acitvity in some cases of BPH may indicated that there are a few of malignant cells, as yet clinically undetected, within the tissue of BPH.