同种骨基质明胶的制备及活性观察

Refining of Allogeneic Bone Matrix Genlatin and Its Activity

目的 观察骨基质明胶 ( BMG)在软组织内植入及与金属材料复合骨组织植入后的活性及作用。方法 取兔四肢骨制作 ,并将其植入 5只兔的腹部肌肉中 ,将 BMG与自蔓延钛镍合金复合后植入 3只兔颅骨中进行观察 ,术后 1、2、3、4、8、12周处死动物 ,进行组织学及电镜观察。结果 BMG植入软组织后 ,3～ 4周时可见软骨基质形成 ,但 8周时 BMG和形成物被完全吸收 ;在硬组织植入 1个月左右 ,复合材料在成骨时间及骨的成熟度上均强于单一金属材料 ,3个月左右则无明显差异。结论 BMG具有异位诱导骨形成的能力 。

Objective To evaluate the characteristics of bone matrix gelatin (BMG) and its effect. Methods The BMG was refined from the legs of rabbits. The BMG was implanted into soft tissues of five rabbits and BMG was compound with NiTi alloys, and then were implanted in the parietal bone of three rabbits. The rabbits were sacrificed at 1,2,3,4,8,12 weeks after implantation. The specimens were prepared and observed by light microscope and SEM. Results Prechondroblasts, cartilage matrix could be observed in soft tissues at 12 weeks and 34 weeks after the BMG implantation respectively. All the BMG and the cartilage were completely absorbed at 8 weeks after the implantation. In the early stage of implantation in the cranium, the compound materials was better than the NiTi alloys in osteoplastic time and maturity of new bone, while in the late stage except the maturity of new bone, there were no significant differences in both compound materials and NiTi alloys. Conclusion The BMG has the capability of ectopy osteoinduction, and played a role in the rapid healing of NiTi alloys with the surrounding bone.