儿童呼吸道非典型病原体的检测及方法评价

Application and Evaluation for the Detecting Techniques of Children Respiratory Atypical Pathogens

目的应用多种检测方法对临床儿童患者非典型呼吸道病原体进行检测,并对检测结果进行分析和方法评价,为临床提供呼吸道感染性疾病病因诊断依据。方法应用IFA法检测儿童常见9种呼吸道病原体的IgM抗体,同时应用GICA法对流感病毒抗原、快速培养法对MP进行检测,并分析比较检测结果。结果 453例样本中,9种IgM抗体总共检出94例,阳性率20.8%。其中应用GICA法检测Flu A和Flu B抗原与IFA法检测抗体总体一致率分别达98.9%和99.6%(Kappa值>0.75,一致性满意),培养法检测MP与PCR法总体一致率达97.6%(Kappa值>0.75,一致性满意)。结论非典型病原体是儿童呼吸道感染的重要病原体,应用IFA抗体检测、流感病毒GICA抗原检测、MP快速培养法能够为临床提供早期诊断和正确治疗的依据。

Objective Use a variety of measurements to detect common respiratory atypical pathogens in clinical children specimens, Evaluate these measurements by analysing the test results, in order to provide diagnosis basis for Respiratory tract infection. Methods 9 types of respiratory atypical pathogens IgM were detected by IFA. Flu was detected by GICA. MP was detected by rapid culture. Results Detection rate of respiratory atypical pathogens was 20. 8% （94） in the 453 samples, via detecting 9 types of respiratory atypical pathogens IgM. The results of GICA detection of Flu have a high consistency of 98. 9% （Flu A） and 99. 6%（Flu B） with that of IFA detection （Kappa〉0. 75）. Rapid culture of MP has a high consistency of 97.6% with that of IFA detection （Kappa〉0. 75） in those samples. Conclusion Respiratory atypical pathogens are important pathogens in respiratory tract infection. Using IFA,GICA and MP rapid culture are able to provide evidence for early diagnosis and proper therapy.