婴幼儿哮喘53例外周血Leptin、Eotaxin、总IgE检测及其临床意义

The expression and clinical significance of the serum Leptin、Eotaxin and TIgE in asthmatic infant and young children

目的:探讨外周血Leptin(瘦素)、Eotaxin(嗜酸粒细胞趋化因子)及TIgE(总IgE)在婴幼儿哮喘发病中的作用及其相互关系。方法:选择53例婴幼儿哮喘患儿及42例健康同龄儿童为研究对象,采用放射免疫分析法检测Leptin、酶联免疫吸附法(ELISA)检测Eotaxin及荧光酶联免疫法检测TIgE水平。结果:①婴幼儿哮喘急性发作期与临床缓解期及健康对照组血清Leptin、Eotaxin及总IgE水平比较均具有显著性差异(P<0.05)。②婴幼儿哮喘患儿急性发作期、临床缓解期Eotaxin与TIgE呈正相关关系(P<0.05);Eotaxin与Leptin、Leptin与TIgE无相关性(P>0.05)。结论:外周血Eotaxin可作为评判婴幼儿哮喘病情严重程度的客观指标;Leptin可能参与婴幼儿哮喘的发病。

Objective： To investigate serum levels of Leptin,Eotaxin and TIgE for effect of nosoenesis and relationship each other of asthmatic infant and young children. Methods： 53 asthmatic infant and young children （study group）and 42 healthy children（control group）were selected. Serum Ieptin was measured by radioimmunity, serum Eotaxin was measured by enzymelinked inmunosorbent assay（ELISA）and serum TIgE was measured by pharmacia with fluoro enzyme-linked immune assay. Results：@The results illustrated that the levels of serum Leptin, Eotaxin and TIgE in study group during the children＇s attack period were all significantly higher than control group, the difference had statistical significance（P〈0.05） ;There were significant difference between attack period and remission period about the levels of serum Leptin in study group （P〈0.05） ②In asthmatic infant and young children, the serum levels of Eotaxin with TIgE was positively correlated（P〈0.05） ;Either Eotaxin or TIgE were not correlated the serum levels of Leptin （P〈0.05）. Conclusion： Both Eotaxin and Leptin may be participated in nosogenesis of asthma ; Eotaxin may be assessment of patient＇s condition.