Syncytin在妊娠高血压疾病胎盘组织中的表达及与围生结局的相关性研究

Syncytin expression in placenta of hypertensive disorders complicating pregnancy and their relation to perinatal outcome

目的探讨胎盘组织中Syncytin的表达在妊娠高血压疾病发病中的作用及与围生结局的相关性。方法采用Westemblot法、免疫组化方法测定syncytin蛋白表达水平与定位，并与围生结局进行相关性分析。结果子痫组、重度子痫前期组、轻度子痫前期组及妊娠期高血压组的Syncytin蛋白表达水平分别为（8．2±0．6）、（9．1±0．4）、（10．8±0．8）及（11．4±0．4），均显著低于正常对照组（20．1±2．2），差异有统计学意义；子痫组与重度子痫前期组差异无统计学意义，轻度子痫前期与妊娠期高血压组差异无统计学意义，其余组别两两之间差异均有统计学意义；Syncytin蛋白定位于胎盘组织滋养细胞的细胞浆内，Syncytin蛋白强阳性反应呈棕黄色；Syncytin蛋白的表达与24h尿蛋白定量、收缩压、舒张压均存在负相关性；Syncytin蛋白表达与产后出血量相关性无统计学意义：各组新生儿体重比较，差异均有统计学意义，新生儿体重与Syncytin蛋白的表达水平无相关关系，但Syncytin蛋白表达有随体重增加而增加的趋势。结论Syncytin可能与妊娠高血压疾病的发病和围生结局相关，可能是疾病发展程度的一个重要表达指标。

Objective To investigate the contribution of placental syncytin expression to the development of hypertensive disorders complicating pregnancy （HDCP） and its correlation with perinatal outcome. Methods Western blot was performed to study the syncytin protein level, followed with correlation analysis with perinatal outcome. Immunohistochemical test was also performed to locate syncytin protein. Results Syncytin protein expression of the HDCP group was as follows., eclampsia （ 8.2 ± 0.6）, severe pre-eclampsia （9.1 ± 0.4）, mild pre-eclampsia （ 10.8 ± 0.8 ）, and pregnancy hypertension （11.4 ± 0.4） ; all were significantly lower than control group （20.1 ± 2.2）. Statistical significance was observed in pair wise comparisons except for eclampsia group versus severe pre-eclampsia group and mild pre-eclampsia group versus pregnancy hypertension group. Syncytin protein was localized at the cytoplasm of trophocyte in placental tissue. Brown madder represented for strong positive reaction in syncytin immunohistochemical test. Significant negative correlation was observed between syncytin level and 24 h urine protein level, systolic pressure, diastolic pressure, while no correlation was noted between protein level and postpartum hemorrhage. Birth weight was not correlated with syncytin protein level, but there was a trend that birth weight increased with syncytin level. Conclusion Our study suggests that syncytin expression may play a role in the etiology of hypertensive disorders complicating pregnancy. Syncytin protein level may be an important index of the development of this disorder and is correlated with perinatal outcome.