摘要
从凡纳滨对虾养殖池塘中采集分离芽孢杆菌、乳酸菌与弧菌,并设置水体碳氮比分别为:C∶Nw=2、5、10、15、20共5个水平。在养殖环境营养水平下研究了芽孢杆菌、乳酸菌与弧菌的生长、拮抗作用及细菌菌体碳氮比(C∶NB)的变化。研究发现,较高的C∶Nw适合芽孢杆菌(C∶Nw=15)与乳酸菌(C∶Nw≥10)的生长,较低的C∶Nw适合弧菌(C∶Nw=5、10)的生长,并且当C∶Nw较高时芽孢杆菌与乳酸菌对弧菌的生长具有明显的拮抗作用。因此向养殖水体和饵料中添加碳源,将环境C∶N提高至超过10的水平不仅可以促进芽孢杆菌与乳酸菌的快速生长,而且可以有效抑制弧菌的繁殖。
From Litopenaeus vannamei culturing ponds, 12 strains of Bacillus, lactic acid bacteria and Vibrio were isolated with their growth, antagonism and cellular C ： N ratio （C = NB） determined in culturing waters with C ： N ratios （C = Nw） of 2, 5, 10, 15 and 20, respectively. We found that Bacillus and lactic acid bacteria grew well when C ： Nw was 15 and 〉10, respectively; while Vibrio grew well when C ： Nw was either 5 or 10. Bacillus and lactic acid bacteria significantly inhibited the growth of Vibrio when C ： Nw was higher than 10 （p〈0.05）. Accordingly, C ： Nw could be adjusted higher than 10 in order to enhance the growth of Bacillus and lactic acid bacteria and inhibit the growth of Vibrio in aquaculture industry.
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2013年第1期34-40,共7页
Periodical of Ocean University of China
基金
国家科技支撑项目(2011BAD13B10)
公益性行业(农业)科研专项(201103034)资助
