穿心莲内酯提高异嗜性粒细胞吞噬和杀伤鸡大肠杆菌O78功能的体外试验

Effect of andrographolide on phagocytic and killing activities of chicken heterophils against Escherichia coli O78 in vitro

模拟大肠杆菌O78在血液中被鸡异嗜性粒细胞吞噬杀伤的过程,利用激光共聚焦和涂板菌落计数的方法研究中药单体成分穿心莲内酯对异嗜性粒细胞吞噬杀伤大肠杆菌O78活性的影响。结果显示,穿心莲内酯质量浓度为1mg/L时有极显著的提高异嗜性粒细胞吞噬大肠杆菌O78的作用(P<0.01),并且在异嗜性粒细胞发挥杀伤作用的12h后有极显著的增强其杀伤作用的功效(P<0.01)。结果表明,穿心莲内酯能够提高异嗜性粒细胞吞噬、杀伤大肠杆菌O78的活性,从而起到增强机体抗病能力,提高机体免疫力的作用。

To study the effect of andrographolide on the phagocytic and killing activities of avian heterophils against Escherichia coli O78,the process of E.coli O78 captured by chicken heterophils in vitro was simulated and examined through laser scanning confocal microscopy and colony counting.The results showed that andrographolide at 1 mg /L could significantly enhance the phagocytic activity of chicken heterophils against E.coli O78（P0.01）.In addition,it could significantly enhance the killing activity of chicken heterophils against E.coli O78 from 12 h after phagocytosis（P0.01）.These results demonstrate that andrographolide can improve the phagocytic and killing activities of chicken heterophils against E.coli O78,which enhance the disease resistance and immunity.