肥胖抑制素在断奶仔猪胃壁中的分布

Distribution of obestatin in gastric wall of weaned piglet

将断奶15d仔猪(45日龄)处死后分别取胃窦部、胃体部、胃底部,4%中性多聚甲醛固定24h后制作组织蜡块,采用SP免疫组化试剂盒和DAB显色法,对各组织蜡块进行免疫组化检测,对检测结果进行拍照并分析。结果显示,肥胖抑制素(Obestatin)在断奶仔猪胃组织内广泛存在,而且在胃的不同部位其分布、含量有所不同。胃底部和胃体部以胞内分泌为主,胃窦部则主要分泌到黏膜外。Obestatin在仔猪胃壁中确实存在,且其在胃壁中的分布从上到下逐渐在组织内和组织外变化。同时,本试验也从侧面证实了猪和其他已证明的动物种属之间的Obestatin具有相似的结构位点。

The experiment was conducted to study the distribution of obestatin in stomach of weaned piglet.Gastric antrum,gastric body,fundus were taken from the piglet which was slaughtered at 15 days after weaning（45 days old）,and immediately fixed in 4% neutral paraformaldehyde for 24 h,then made paraffin-embedded tissue for immunohistochemical staining using SP immunohistochemistry kit and DAB staining kit,and results of tests were photographed and analyzed.Results showed that obestatin was widespread in the stomach tissue of piglet.Its distribution and content of secretion was different.Mainly it was located in fundus and gastric body,and secreted into outside of the mucosa in gastric antrum.Obestatin did exist in stomach of weaned piglet.And its distribution gradually changed within and outside of the organization from top to bottom in the stomach.