牛磺鹅去氧胆酸对糖皮质激素引起小鼠免疫抑制的影响

Effect of taurochenodeoxycholic acid on the immunosuppression mice with glucocorticoid

采用80mg/kg剂量的地塞米松对小鼠进行腹腔注射制备免疫抑制小鼠模型;牛磺鹅去氧胆酸(Tauroche-nodeoxycholic acid,TCDCA)设计了0.05、0.10、0.20g/kg低、中、高3个给药剂量对小鼠灌胃给药;检测免疫器官质量,碳粒廓清法检测单核-巨噬细胞吞噬功能,流式细胞术检测外周血CD4+和CD8+细胞亚群百分率及CD4+与CD8+的比值,ELISA检测血清中IgG的含量。结果显示:模型小鼠和正常小鼠相比较,脾指数、单核-巨噬细胞吞噬功能、CD4+和CD8+亚群百分率及血清IgG含量均极显著降低(P<0.01),说明地塞米松制备的小鼠模型免疫功能极显著低下。给药组与模型组相比较,TCDCA的3个剂量组对小鼠脾指数均无显著影响(P>0.05);低剂量组和中剂量组极显著增强单核-巨噬细胞系统吞噬功能(P<0.01);3个剂量组均显著提高外周血中CD4+细胞百分率(P<0.05),中剂量组显著提高CD4+与CD8+的比值(P<0.05);低剂量组和中剂量组显著提高小鼠血清中IgG含量(P<0.05)。结果表明,TCDCA对糖皮质激素免疫抑制小鼠的非特异性免疫和特异性细胞免疫、体液免疫功能具有恢复作用,但对脾脏萎缩无恢复作用。

The immunocompromised model mice was preparated with 80 mg/kg dexamethasone using peritoneal injection,three doses of TCDCA were intragastriced to the immunosuppression mice,including 0.05（low-dose group）,0.1（middle-dose group）,0.2（high-dose group）g/kg;the weight of the immune organs were measured,the phagocytosis of the mononuclear phagocyte system was determined using carbon particle clearance test,the percentage of CD4＋ and CD8＋ and CD4＋/CD8＋ ratio in the blood cell were measured using flow cytometry and the content of IgG in serum was assayed using ELISA.The results showed that spleen index,mononuclear macrophage phagocytic function,CD4＋ and CD8＋ subpopulations percentage and the serum IgG content were significantly decreased（P0.01） comparing model mice with normal mice;it illustrated that immune function is significantly low in the model mice.Compared with model group,three doses of TCDCA no significant impact to the index spleen（P0.05）;low-dose group and middle-dose group could significantly improve the phagocytic function of the mononuclear phagocyte system（P0.01）;three doses of TCDCA could enhanced the percentage of CD4＋ lymphocytes（P0.05） and middle-dose group could increase CD4＋/CD8＋ ratio in peripheral blood（P0.05）;the content of serum IgG in mice was enhanced in low-dose group and middle-dose group（P0.05）.These results suggested that TCDCA can significantly enhance the nonspecific immunity,cellular and humoral immunity function of the immunosuppression mice with glucocorticoid.But for splenic atrophy without recovery function.