摘要
目的探讨慢性丙型肝炎(CHC)患者外周血中CD4+CD25+调节性T细胞含量与HCV-RNA的相关性。方法用流式细胞仪检测80例CHC患者和30例健康对照者外周血CD4+CD25+Treg细胞表达,荧光定量PCR检测HCV-RNA含量。结果 CHC患者外周血中CD4+CD25+Treg细胞明显高于健康对照组(P<0.01),且随HCV-RNA载量增高,患者外周血中CD4+CD25+Treg细胞的含量逐渐升高(P<0.01)。CD4+CD25+Treg细胞与HCV-RNA取对数后呈正相关(r=0.588,P<0.01),与ALT呈正相关(r=0.306,P<0.01)。结论 CHC患者外周血中CD4+CD25+Treg细胞增高,且与HCV-RNA含量及ALT升高具有一致性。
Objective To explore the amount of CD4 ^+ , CD25 ^+ regulatory T cells in peripheral blood of patients with chronic hepatitis C ( CHC ) and to study the relationship between these cells and HCV - RNA load. Methods The amount of CD4 ^+ , CD25 ^+ regulatory T cells in peripheral blood of 80 patients with chronic hepatitis C (CHC) and 30 healthy controls were detected by flow cytometry. The load of HCV - RNA was detected by fluorescent quantitative polymerase chain reaction ( FQ - PCR) assay. Results The amount of CD4 ^+ , CD25 ^+ regulatory T cells in peripheral blood of patients with chronic hepatitis C was significantly higher than those of control group ( P 〈0.01 ), and the amount of CD4 ^+ , CD25 ^+ regulatory T cells in peripheral blood of patients was gradually increased following the increase of HCV - RNA load ( P 〈 0.01 ). There was a positive correlation between amount of CD4 ^+ , CD25 ^+ regulatory T cells and HCV - RNA load after taking logarithm ( r =0. 588, P 〈0.01 ), and it was same as ALT ( r = 0. 306, P 〈 0.01 ). Conclusion The increase in amount of CD4 ^+ , CD25 ^+ regulatory T cells in patients with CHC is in accordance with copies of HCV DNA and increased level of ALT.
出处
《临床和实验医学杂志》
2013年第1期18-19,共2页
Journal of Clinical and Experimental Medicine