IL-4、IFN-γ在特发性肺纤维化患者血清中的检测及意义

Detection and significance of serum levels of IL-4 and IFN-γ in patients with idiopathic pulmonary fibrosis

目的探讨血清白细胞介素(IL-4)、干扰素(IFN-γ)水平在特发性肺纤维化(IPF)发生发展中的意义。方法应用双抗体夹心酶联免疫吸附试验法(ELISA)方法对27例IPF患者治疗前与治疗3个月后有效组(n=18)和无效组(n=9)外周血血清中的IL-4、IFN-γ含量进行检测分析。结果 IPF患者血清中的IL-4水平高于健康对照组(P<0.05),血清IFN-γ水平低于健康对照组(P<0.05)。血清IL-4水平治疗后有效组较治疗前降低(P<0.05),无效组较治疗前升高(P<0.05),血清IFN-γ水平治疗后无效组比治疗前降低(P<0.05)。结论 IPF的病情进展与患者血清中IL-4水平增高或者IFN-γ的水平降低有关,检测血清中IL-4、IFN-γ含量变化特点有助于了解其与IPF的关系,可能进一步作为IPF辅助诊断的有效指标。

Objective To investigate the significance of the serum levels of interleukin - 4 （ IL - 4 ） and interferon - γ （ IFN - γ） in idio- pathic pulmonary fibrosis （IPF） pathogenesis. Methods Double -antibody Sandwich ELISA was applied to detect IL-4 and IFN -γ levels in peripheral blood serum of 27 IPF patients prior to and 3 months after treatment. Results Compared to the control group, IL -4 level in IPF patients was higher （ P 〈 0. 05 ） , while IFN - γ level was lower （ P 〈 0. 05 ）. IL - 4 level was decreased in patients after effective treatments （ P 〈 0.05 ）. and increased in those with noneffective treatments （ P 〈 0.05 ）. However, IFN - γ level was decreased in patients after noneffective treatments （ P 〈 0. 05 ）. Conclusion The high level of IL - 4 or the low level of IFN - γ may relate with IPF pathogenesis. The changes of the levels of IL - 4 and IFN - γ can be served as auxiliary diagnostic markers of IPF.