大鼠大脑皮质梗塞后显微激光拉曼光谱的变化

LASER RAMAN MICROSPECTROSCOPY OF INFARCTEDCEREBRAL CORTEX IN RATS

用Spex—1428显微激光拉曼光谱仪测定局灶性脑梗塞后大鼠大脑皮质的激光拉曼光谱的变化 ,以正常侧大脑皮质作对照 ,研究大鼠大脑皮质梗塞后生物大分子的结构及化学成分的变化 ,得到如下结果 :(1)特征峰1076cm-1 增大 ,表明梗塞区的DNA的结构出现变化 ,其有序性降低 ;(2)1572cm -1为与蛋白质侧链有关的特征峰 ,脑梗塞后此峰明显降低 ,表明梗塞区的蛋白质结构亦出现变化 ;(3)832cm -1(Tyr)峰降低 ,表明梗塞区的酪氨酸含量减少 ;(4)梗塞区出现922cm -1和946cm-1 两个新特征峰 ,表明梗塞区的羧酸(COOH)含量增加。结果显示 :脑梗塞后大鼠大脑皮质的生物大分子的结构及化学成分均出现明显的变化 ,与脑梗塞的病理变化结果密切相关。

Spex-1428 laser Raman microspectroscopy was used to analyze the changes of the biomolecular structure of the infarcted cerebral cortex in rats. Results are as follows. (1). The peak intensity of 1076cm-1 is markedly increased after cerebral infarction. It means that the orderness of DNA chains is markedly decreased.(2). The peak intensity of 1572cm-1 is markedly decreased after cerebral infarction. It indicates that the structure of the protein molecules is also changed after cerebral infarction.(3).The Raman peak of tyrosine (832cm-1) is significantly decreased after cerebral infarction. It means that the levels of some neurotransmitters such as dopamine and noradrenaline(derivatives from tyrosine) are also markedly decreased. (4). Two new Raman peaks(992cm-1 and 946cm-1) appear in the infarcted cerebral cortex which indicate that the content of carboxylic acid (COOH) is increased in that region. It is concluded that biomolecular structure and chemical components are markedly changed in the infarcted cerebral cortex. These findings are closely correlated to the pathological changes of the infarcted cerebral cortex.