摘要
为验证C60 对活体肿瘤的光动力学损伤作用,我们从两方面进行实验 :C60 对荷瘤小鼠的S180实体瘤的光动力学杀伤作用和C60 对离体S180 肿瘤细胞的光动力学杀伤作用。在小鼠的瘤体上注射C60 光敏剂 ,在511nm和578nm混合黄绿色激光照射下 ,激发C60,产生大量的单线态氧 ,杀伤活性肿瘤。在激光光强为500mW ,C60 浓度为30μg/ml时 ,荷瘤小鼠寿命平均延长5天 ,瘤径减小1cm,瘤重减轻0.8克 ,在活体水平上验证了C60 的光动力学作用 ;另外,在离体的S180 肿瘤细胞中加入细胞培养物和C60 脂质体光敏剂 ,用碘钨灯照射激发C60。经MTT法和FDA -PI双色荧光分光光度法检测 ,离体S180 肿瘤细胞也明显被杀伤。C60 具有强的光敏剂作用 ,它不仅在活体水平上可以杀伤S180 实体瘤而且对离体的S180 实体瘤细胞有强的杀伤作用。
To prove C60 photodynamic effect on tumor cell, i.e. the photodynamic effect on mouse S180 tumor in vivo and the photodynamic effect on S180 tumor cells in vitro we injected the C60-liposome into the mouse S180 tumor body. We found that with green laser(511nm & 578nm)illumination the mouse S180 tumor body was strongly damaged. Under the intensity of 500mW and the concentration of C60(30ug/ml),mouse average survival time extended 5 days and the turmor size reduced 1cm and tumor weight decreased 0.8g. In the other way S180 tumor cells was co-cultured in vitro with C60-liposome for two days. The result of the killing was examed by MTT method and FDA-PI double staining method after illuminating the C60 with I-W lamp. S180 tumor cell in vitro were obviously damaged. C60 is a kind of strong hotosensitizer. It can kill mouse S180 tumor cells both in vivo and in vitro.
出处
《生物物理学报》
CAS
CSCD
北大核心
2000年第2期401-405,共5页
Acta Biophysica Sinica
