东北红豆杉细胞两液相培养中紫杉醇释放行为研究

Study on Taxol Release in the Two-liquid-phase Cultures of Taxus cuspidata

在东北红豆杉细胞悬浮培养中 ,分别研究了稀土化合物 (硫酸铈铵 )、有机溶剂 (油酸和邻苯二甲酸二丁脂 )和稀土化合物与有机溶剂的协同作用对紫杉醇释放的影响。在此基础上深入研究了在东北红豆杉细胞两液相培养中 ,紫杉醇释放率随不同的有机溶剂 (烷烃、有机酸、醇和脂 )、有机溶剂的体积分数、有机溶剂的加入时间和有机溶剂相毒性的变化规律。结果表明分别加入稀土化合物和有机溶剂都明显促进紫杉醇的释放 ,特别是有机溶剂更显著促进紫杉醇的释放。但在东北红豆杉细胞两液相培养中 ,稀土化合物加入不能进一步促进紫杉醇的释放。因此两液相培养中有机溶剂本身就是很好的产物释放剂。紫杉醇的释放率由对照组的 40 %提高到 75 %以上。

Effects of rare earth compound (ammonium sulphate),organic solvents(oleic acid and dibutylphthalate)and the integrated function of the rare earth compound and organic solvents were studied on taxol release in the Taxus cuspidata suspension cultures.And then effects of different organic solvents(paraffin,organic acid,alcohol and ester),their volume tric fraction and phase toxicity were studied on taxol release in the two liquid phase cultures of Taxus cuspidata. The results showed that the addition of the rare earth compound or the organic solvents could strengthen obviously taxol release,especially the organic solvents.But the addition of the rare earth compound could not strengthen further taxol release in the twoliquid phase cultures of Taxus cuspidata. Therefore the organic solvents were very good permeabilizing reagents,which could enhance obviously secondary metabolite in the twoliquid phase cultures of plant cells.Release percentage of taxol was increased into more than 75% from 40% of the control.