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应用动力学方法在线检测Vero细胞培养过程中的摄氧率 被引量：4

On-line Measurement of Oxygen Uptake Rate in the Cultivation of Vero Cells Using the Dynamic Method
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摘要 The oxygen uptake rate( OUR ) during the cultivation of Vero cells in 1.5L CelliGen bioreactor was on line determined using the dynamic method.The results showed that the cell growth and metabolic state during the exponential growth phase was lineally related to the OUR .This implies that the on line measurement of OUR can be used to promptly monitor the physiological state of cultured cells and to efficiently avoid contamination because of frequent sampling in the large scale cultivation of mammalian cells. The oxygen uptake rate( OUR ) during the cultivation of Vero cells in 1.5L CelliGen bioreactor was on line determined using the dynamic method.The results showed that the cell growth and metabolic state during the exponential growth phase was lineally related to the OUR .This implies that the on line measurement of OUR can be used to promptly monitor the physiological state of cultured cells and to efficiently avoid contamination because of frequent sampling in the large scale cultivation of mammalian cells.
作者 周亚竞 谭文松 赵佼 华平 孙祥明 俞俊棠
机构地区 华东理工大学生物反应器工程国家重点实验室
出处 《生物工程学报》 CAS CSCD 北大核心 2000年第4期525-527,共3页 Chinese Journal of Biotechnology
基金 上海市启明星计划的资助
关键词 VERO细胞 在线检测 摄氧率 细胞培养 动力学方法 Vero cell, on line measurement, OUR
分类号 Q813.1 [生物学—生物工程]
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生物工程学报
2000年 第4期

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