质粒pMC71A-LEU转化阴沟肠杆菌E26-1的研究

TRANSFORMATION OF Enterobacter cloacae E26-1 WITH PLASMID pMC71A-LEU

质粒pMC71A－LEU在对阴沟肠杆菌E26－1（EnterobactercloacaeE26－1）的转化中研究了感受态细胞、CaCl2浓度、热激温度和质粒DNA量对转化频率的影响。结果表明，在阴沟肠杆菌F26－1生长到OD600＝0．45时，用50mmol／LCaCl2处理细胞，加入100ng质粒DNA，在32℃热激2min，转化频率最高，可达1×103转化子／μgDNA。

The effect of plasmid pMC71A-LEU to receptive cells, concentrtion of CaCl2, heat stimulated temperature and the arnotint of plasmid DNA in transformation frequency of Enterobacter cloacae E26-1 was studied. The results indicated that when E. chacae grew at OD600 = 0. 45, the transformation frequency was the highest, as high as 1 × 10 3 transformants/μg,when the cells treaed with 50 mmol/L of CaCl2, adAn with 100ng of plasmid DNA, and heat stimulation at 32 ℃ for 2 minutes.