人胎蓝斑神经元免疫组织化学研究

IMMUNOHISTOCHEMICAL STUDIES ON THE NEURONS OF LOCUS COERULEUS IN HUMAN FETUS

为了探讨人蓝斑神经元的胚胎发育特征 ,为蓝斑 -脊髓移植选择适宜胎龄提供形态学根据 ,本研究用免疫组织化学技术系统地观察了人胎蓝斑酪氨酸羟化酶样免疫反应阳性神经元的发育。结果证明 :( 1)蓝斑酪氨酸羟化酶样神经元在胎龄 4个月时已经出现在蓝斑的腹侧部 ;( 2 )蓝斑酪氨酸羟化酶样神经元随胎龄增长逐渐增多 ,以 5个月时增加显著 ;( 3)酪氨酸羟化酶样神经元的密度在胚胎早期升高 ,晚期呈下降趋势 ;( 4)酪氨酸羟化酶样神经元主要分布在蓝斑的背侧部 ,少量散在于腹侧部 ;( 5)酪氨酸羟化酶样神经元开始出现时呈圆形或卵圆形 ,5～ 6个月时呈锥形和梭形 ,7～ 8个月时则以梭形、多角形为主。其胞体逐渐增大 ,胞浆逐渐增多 ,核浆之比由大变小 ,胞突从粗短变为细长平滑。本研究结果提示 ,人胎蓝斑移植以

To explore the development of locus coeruleus neurons in human fetus and provide morphological basis to select embryo locus coeruleus transplanted into transected spinal cord, the neurons of locus coeruleus in human fetus was studied with tyrosine hydroxylase (TH) immunohistochemical method. The result was: (1) The neurons containing TH were found at the fourth month embryo in ventral part of locus coeruleus; (2) The number of TH neurons increased gradually with fetal age, espesially obviously in the fifth month embryo; (3) The density of the TH neurons increased in early embryo and decrease gradually with fetal age in later stage of embryo; (4) The neurons containing TH were mainly located in the dorsal part of locus coeruleus, a few larger neurons were part of located in the ventral part of locus coeruleus; (5) Most of TH neurons were round or oval at the fourth month; mainly pyramidal and spindle from the fifth to sixth month and spindle and angular in the seventh to eighth month embryo. The size of the cell bodies and the cytoplasm of neurons increased gradually with fetal age, the ratio of nucleus to cytoplasm became smaller with fetal age. The cytoplasm processes changed from short, broad and rough in surface into long, thin and smooth. It is concluded that the neurons of locus coeruleus at the fourth month embryo are more suitable for graft.