羟乙基淀粉130/0.4的C2/C6置换比例的GC-MS和GC-FID法测定结果比较

Comparison of GC-MS and GC-FID Results for the Determination of C2/C6 Substitution Ratio of Hydroxyethyl Starch 130/0.4

将羟乙基淀粉130/0.4相继用酸水解、六甲基二硅氧烷衍生化后,分别采用GC-MS和GC-FID法测定并比较羟乙基淀粉C2位和C6位的羟乙基置换比例。结果显示,GC-FID法重复性好、进样精密度高,结果可靠;由于存在C2位和C6位衍生物离子化效率的差异,GC-MS法不适合用作该项目的定量方法,但可作为辅助的色谱峰定性工具。

Hydroxyethyl starch 130/0.4 was hydrolyzed by acid and trimethylsilylized by hexamethyl disiloxane successively. The resulting sample solution was utilized to determine C2/C6 substitution ratio by GC-MS and GC-FID method. The results showed that the GC-FID method had high repeatability, precision and reliability. However, the GC- MS method was unsuited for the quantitative analysis of C2/C6 substitution ratio, due to the discrepancy in ionization efficiency of the C2- and C6-derivatives. Nevertheless, it was a supplementary tool for identifying chromatographic peaks.