宁夏回族代谢综合征与脂联素水平及基因多态性的相关性研究

Relation of metabolic syndrome with plasma adiponectin level and gene polymorphism in Ningxia Hui subjects

目的探讨脂联素水平及其单核苷酸多态性(SNP+45T/G和SNP+276G/T)2个位点与宁夏回族代谢综合征(MS)的关系。方法选择祖居宁夏、无亲缘关系、3代内无异族通婚史的回族个体共305例,分为MS组207例和对照组98例;应用ELISA及PCR-RFLP技术检测血浆脂联素水平及其2个位点的SNP。结果与对照组比较,MS组体重指数、腰臀比、收缩压、舒张压、空腹血糖、空腹胰岛素、TG、LDL-C、胰岛素抵抗指数明显升高,TC、HDL-C、脂联素水平明显降低,差异有统计学意义(P<0.05,P<0.01)。与对照组比较,MS组SNP+45位点TG+GG基因型和G等位基因频率明显升高,TT基因型及T等位基因频率明显降低,差异有统计学意义(P<0.01)。多因素逐步logistic回归分析显示,SNP+45T/G基因多态性是回族MS的危险因素。结论回族MS患者脂联素水平下降,回族人群中携带SNP+45G等位基因者患MS的风险增加。

Objective To study the relation of metabolic syndrome with plasma adiponectin level and mononucleotide polymorphisms at SNP45T/G and 276G/T in Ningxia Hui subjects. Methods Three hundred and five Hui subjects from Ningxia Hui Autonomous Region with no genetic rela- tionship and interracial marriage in 3 generations, were divided into metabolic syndrome group （n=207） and control group（n=98）. Their plasma adiponectin level was measured by ELISA and their gene polymorphisms at SNP45T/G and 276G/T were detected by PCR-RFLP. Results The body mass index,waist/hip ratio,systolic blood pressure,and diastolic blood pressure,plasma levels of adiponectin, fasting blood glucose and insulin, TG, TC, LDL-C were significantly higher whereas the plasma HDL-C level was significantly lower in metabolic syndrome group than in control group（P〈0.05,P〈0.01）. The frequencies of genotypes at SNP＋45 TG＋GG and G alleles were significantly higher while those of TT genes and T alleles were significantly lower in metabolic syndrome group than in control group（P〈0.01）. Multivariate logistic regression analysis showed that the gene polymorphisms at SNP＋45T/G were the risk factors for metabolic syndrome in Hui subjects. Conclusion The adiponectin level is lower in Hui metabolic syndrome patients. The risk to develop metabolic syndrome increases in Hui subjects carrying SNP＋45G alleles.