黄伞菌种培养基配方筛选研究

The Selection of Spawn Culture Medium Formulas for Pholiota adiposa

选用9种母种培养基配方和11种原种培养基配方,研究了不同的菌种培养基对黄伞菌丝生长的影响;经过预备试验,观测黄伞菌丝的生长情况,以期筛选适合黄伞生长的母种和原种培养基配方。结果表明:不同配方的母种、原种培养基上菌丝生长存在显著差异,黄伞菌丝生长要求较低的氮浓度和较高的碳氮比。其中最适合黄伞菌丝生长的母种培养基为配方2和配方4,在生产上,配方2、4、1均可选用。适合黄伞菌丝生长的原种培养基为配方1、5、6。其中,配方1为麦粒培养基,成本较高,其中的菌丝老化较快,在生产上急需菌种时可选用。最适合生产上推广的原种培养基为配方5,不仅原材料来源广泛,成本较低,而且菌丝生长速度快,老化慢,较耐贮存。

In order to obtain optimum stock and pre-cuhure spawn culture reed/urn formulas of Pholiota adiposa, the effects of different culture mediums on mycelia growth of Pholiota adiposa were studied. After preliminary experiment, 9 stock culture medium formulas and 11 pre-culture spawn medium formulas were used to observe the mycelia growth status. The results showed that there were significant differences of the mycelia growth rate and growth potential on different culture mediums. The mycelia growth of Pholiota adiposa need lower nitrogen content and higher carbon nitrogen ratio of cultivation materials. The optimal stock culture mediums were formulas 2 and 4. Each of formulas 2,4 and 1 could be used for Pholiota adiposa cultivation. The pre-culture spawn mediums suitable for Pholiota adiposa mycelia growth were formulas 1,5 and 6. Formula 1 of wheat grain medium cost higher, and mycelia grown in it aged faster than in other mediums. The wheat grain medium could be used when spawn production was pressed for time. The optimum selection of pre-euhure spawn medium was formula 5 for Pholiota adiposa cultivation. The mycelia grew fast and aged slowly in it,while it had the advantages of abundant raw material and reasonable prices.