髓复康对大鼠脑缺血损伤区内勿动蛋白(NoGo-A)表达的影响

Effect of Chinese Herb Suifukang on Expression of NoGo-A Protein in the Brain of MACO Rat

目的研究益气活血补肾方髓复康对大鼠脑缺血损伤区勿动蛋白(NoGo-A)表达的抑制作用。方法通过Koizumi法制作SD大鼠单侧大脑中动脉阻塞模型(MACO),将SD大鼠随机分为正常组、模型组、阳性对照组、髓复康大剂量组、中剂量组和小剂量组,各组又分别在给药8、15和30 d 3个时间点取大鼠脑组织,通过免疫组化、RT-PCR的方法检测各组脑缺血损伤区勿动蛋白表达量的差异。结果中药大剂量及中剂量组勿动蛋白表达最弱,均与模型组形成极显著差异(P<0.001),小剂量组的勿动蛋白表达也低于模型组,与之形成显著差异(P<0.05)。结论髓复康可以抑制脑缺血损伤区勿动蛋白的表达,改善脑缺血损伤区轴突再生微环境,可能是其促进脑缺血损伤后轴突再生的机制之一。

OBJECTIVE To study the effect of Chinese herb Suifukang on the expression of Nogo protein-A in the brain of MCAO rats and explore the action mechanism of its promoting neurogenesis. METHODS SD rats were selected and randomized into six groups ： normal group, control group, treatment group of methylprednisolone, treatment group of Chinese herb Suifukang in large-does, middle-does and small-does. The models of MCAO rats were successfully established by the method of Koizumi. After the surgery, the rats of drug group had been taken orally Suifukang by 10 g · kg-1 crude drug one time daily, and the rest groups had been taken orally equivalence saline once a day. The brain of rats were investigated and the expression of NoGo-A was detected by immunohistology method and RT-PCR method. RESULTS The expression of NoGo-A in the injured brain was apparently inhibited by Suifukang（P 〈 0. 01 ）. CONCLUSION Suifukang can inhibit the expression of NoGo-A. So this function may be one of the mechanisms that Suifukang can promote the functional recovery after brain injury.