几种常见果蔬抗氧化活性与多酚和维生素C的关系

Sutdy on the antioxidant activity of several common vegetables and fruits and the correlation between the content of pdyphenols and V_C and the antioxidant activity

目的:探索几种常见果蔬的抗氧化活性与多酚、维生素C的相关关系。方法:运用二苯代苦味酰基自由基法(DPPH法)、硫代巴比妥酸反应物法(ABTS法)、血浆高铁还原能力分析能力(FRAP法)、羟自由基和超氧阴离子自由基5种抗氧化方法评价10种常见果蔬的抗氧化活性,并采用Folin-Ciocalteu法和2,4-二硝基苯肼法分别测定了果蔬中多酚和VC的含量,并对多酚、VC的含量与抗氧化活性进行相关性分析。结果:DPPH、ABTS、FRAP、超氧阴离子、羟自由基抗氧化活性最强的依次为豆角、豆角、橘子、香蕉、橘子,最弱的依次为西瓜、西瓜、西瓜、胡萝卜、卷心菜。DPPH、ABTS和FRAP测定果蔬粗提液抗氧化活性与多酚的含量的相关性强于VC。结论:多酚的含量对果蔬抗氧化活性的影响强于V_C。

Objective：To explore the relationship of the antioxidant activity and total polyphenols and Vitamin C（VC）in several fruits and vegetables.Methods：2,2-Diphenyl-1-picrylhydrazyl（DPPH）2,2-azino-bis-（3-ethylbenzoth iazoline-6-sulphonic acid）（ABTS）,Ferric Reducing Antioxidant Potential assay（FRAP）,Hydroxyl Radicals and Superoxide anion Radical were used to evaluate the antioxidant activity of ten fruits and vegetables.The Folin-Ciocalteu method and 2,4dinitrophenylhydrazine method were used to determine the content of the total phenols and VC.We also make the correlation analysis between the content of polyphenols and VC and the antioxidant activity.Results：In the five methods（DPPH,ABTS,FRAP,Superoxide anion Radical and Hydroxyl Radicals）,string beans,string beans,orange,banana and orange had the strongest antioxidant activity in order,and the weakest was watermelon,watermelon,watermelon,carrot,cabbage in order.Antioxidant activity that determined by DPPH,ABTS FRAP about the fruit and vegetable coarse extraction liquid had the stronger correlation with polyphenols than VC.Conclusion：The content of polyphenols was stronger than VC in the influence of the fruit and vegetable antioxidant activity.