响应面法优化留胚精米中积累γ-氨基丁酸的发芽条件

Research on optimization of GABA content accumulation during the germ-remaining milled rice germination by response surface methodology

以留胚米Z601为原料,利用发芽过程使精米中γ-氨基丁酸积累。通过单因素实验、响应面实验得到了精米中GABA含量与浸泡温度、浸泡时间、发芽温度、发芽时间4种发芽条件回归模型,确定了对GABA含量积累最有利的发芽工艺条件为浸泡温度31℃、浸泡时间13h、发芽温度31℃、发芽时间27h,此条件下精米中GABA含量为31.13mg/100g,是不发芽条件下的4倍。

Germ-remaining rice Z601 was used as material to accumulate GABA content during germination.A quadratic polynomial model was established for GABA content as a function of four variables which were soaking temperature and time as well as germination temperature and time.The optimum conditions were as follows：soaking temperature at 31℃,soaking time at 13h,germination temperature at 31℃,germination time at 27h.Under the optimum condition,GABA content reached 31.13mg/100g,which was almost 4 times higher than that in the rice which did not germinate.