荞麦和商品苦荞茶中总黄酮的含量测定

Determination of total flavonoids in buckwheat and buckwheat goods

目的：测定荞麦不同栽培种、植株不同部位以及不同商品苦荞茶中的总黄酮含量。方法：采用UV法测定总黄酮含量;通过回流法提取,NaNO3-Al（NO3）3法显色测定样品吸光度,以芦丁为标准溶液计算求得总黄酮含量。结果：芦丁在0.003006~0.1503mg/mL浓度范围内线性关系良好,R2=0.9999;平均回收率为97.9%,RSD为1.95%。结论：不同栽培种荞麦总黄酮含量测定中,苦荞的总黄酮含量在13.67~21.56mg/g之间,甜荞总黄酮含量在5.79~7.23mg/g之间,苦荞总黄酮含量总体高于甜荞;荞麦植株不同部位中叶的总黄酮含量最高,最高达到68.30mg/g;商品苦荞茶中,所测商品总黄酮含量在6.96~16.78mg/g之间,较苦荞种子中总黄酮含量有一定程度减少。该方法简便快捷,结果准确,重现性好,适用于荞麦和商品苦荞茶中总黄酮含量的测定。

Objective：To determine the content of total flavones in the different kinds of buckwheat,different parts of buckwheat and buckwheat goods. Method：UV spectrophotometry for determination of total flavonoids was used. Using Na （NO 3 ） -Al （NO 3 ） 3 to detect the absorbance of samples and then count the total flavonoids. Results：The linear range of rutin was 0.003006~0.1503mg/mL （R 2 =0.9999） with average recovery of 97.9% （RSD 1.95% ）. Conclusion：In different cultivated species of buckwheat,the concentration of total flavonoids of Fagopyrum tataricum（L.） Gaerth was 13.67~21.56mg/g,and that of F. esculentum Moench was 5.79~7.23mg/g. In different parts of buckwheat,the concentration of total flavonoids of leaves was the highest,up to 68.30mg/g. For buckwheat tea,the concentration of total flavonoids was 6.96~16.78mg/g,but they were lower than that of buckwheat seeds. This method was simple,accurate and reproducible for determination of total flavonoids of buckwheat and buckwheat goods.