发光费氏弧菌对常用色素的生物毒性评价

Application of luminescent Vibrio fischeri to evaluation of toxicity of several common pigments

在探寻出色素与费氏弧菌发光抑制率存在相关性的基础上,初步建立起评价色素生物毒性的方法,并将其应用于果汁中色素产生的综合毒性的检验。结果:色素溶液质量浓度与发光抑制率呈正相关,相关系数在0.8760～0.9873之间,EC50范围在0.72～2.86mg/L之间;果汁中色素质量浓度与发光抑制率同样呈正相关,相关系数在0.9136～0.9951之间,EC50值在1.02～2.96mg/L之间。柠檬黄毒性较小,苋菜红毒性较大。果汁对人工合成色素的生物毒性具有一定影响。色素在果汁中对费氏弧菌发光抑制率随浓度增加而上升的幅度明显大于在水溶液中。本研究为食品急性生物毒性监测的研究提供初步参考。

A method built on the basis of the toxicity effect of pigments on luminescent bacterium Vibrio fisheri was developed for determining the toxicity and content of pigments. Results showed that there was a positive correlation between inhibitory rate of pigments against luminescent intensity of Vibrio fisheri and pigments concentration in aqueous solution,with a correlation coefficient ranging within 0.8760~0.9873. EC 50 range was 0.72 ~2.86mg/L. Inhibitory rate of pigments against luminescent intensity of Vibrio fisheri was also positively correlated with positive concentration in fruit juice,and the correlation coefficient was between 0.9136 and 0.9951. EC 50 range was 1.02~2.96mg/L. This showed that the toxicity of tartrazine was lower and the toxicity of amaranth was larger. Fruit juice played a role of mitigating the toxicity of the synthetic pigments. The bioluminescence inhibition rate by the pigments in the fruit juice increased with the rate significantly was higher than in aqueous solution. The study provided the initial reference for the acute toxicity testing of the food.