响应面分析法优化乳白香青中绿原酸提取工艺

Optimization of extraction technology of chlorogenic acid from Anaphalis lactea Maxim. by response surface methodology

以乳白香青为原料,确定乳白香青中绿原酸最佳提取的工艺条件。在单因素实验的基础上,运用Box-Behnken中心组合实验设计,建立绿原酸提取得率的二次回归模型,确定乳白香青中绿原酸的优化工艺条件为:提取温度为41℃,液料比为31∶1(mL/g),甲醇浓度为66%(v/v),绿原酸的得率为5.2796(mg/g),接近于模型预测值5.3295(mg/g)。

In order to promote the extractability of chlorogenic acid from Anaphalis lactea Maxim,we established a quadric regression equation for predicting the yield of chlorogenic acid on the basis of one-factor tests, according to Box-Behnken design . The result showed that the optimum conditions for the extraction of chlorogenic acid were as follows：extraction temperature 41℃ ,ratio of material to solvent 31∶1 （mL/g）,Mthanol concentration 66%（v/v）. Under these conditions,the chlorogenic acid yield was up to 5.2796 （mg/g） which was well matched with the predictive extracting yield 5.3295（mg/g）.