D-101大孔树脂分离纯化两面针总生物碱

Separation and purification of total alkaloids from Zanthoxylum nitidum(Roxb.)DC.with D-101 macroporous resin

目的研究大孔树脂分离纯化两面针总生物碱的工艺。方法用UV测定总生物碱含量来优化工艺参数,用TLC分析分离效果。结果以体积分数60%乙醇-5g·L-1盐酸提取液上D-101大孔树脂柱,流出液回收乙醇至45%,再上D-101大孔树脂柱,其流出液再回收乙醇至无醇味,再上D-101大孔树脂柱,分别用体积分数95%,80%和50%乙醇洗脱。产品总生物碱质量分数为18.07%～27.67%。结论该法既富集了总生物碱,又初步分离了不同极性成分。

Objective To explore a process to separate and purify total alkaloids from Zanthoxylum nitidum（Roxb.）DC.with macroporous resin.Methods UV was used to determine the total alkaloids while TLC was used to analyze the separation efficacy.Results Extract of volume fraction 60% alcohol-5 g·L-1 hydrochloric acid was absorbed and separated with D-101 macroporous resin.Then the alcohol in the effluent was recycled and reduced to 45%,and the concentrated solution was absorbed and separated with the resin.Finally,the alcohol in the effluent was recycled and reduced to no alcohol flavor,and the concentrated solution was absorbed and separated with the resin.Total alkaloids were eluted with 95%,80% and 50% alcohol,respectively.Mass fraction of total alkaloids in products was 18.07%-27.67%.Conclusion The process not only enriches total alkaloids,but also separates simply ingredients with different polarity.