正交实验法优选川芎白芍药对的合提工艺

Optimization of the extraction conditions of the couple herbs,Radix paeoniae Alba and Rhizoma chuanxiongby orthogonal experiment design

目的优选川芎、白芍的乙醇合提工艺。方法采用正交实验设计,以没食子酸、芍药苷、阿魏酸含量为指标,以乙醇体积分数、加醇量、煎煮时间、煎煮次数为考察因素,筛选最佳提取条件。结果最佳提取工艺为:体积分数50%乙醇,12倍药材量,煎煮2次,每次2h,所得没食子酸、芍药苷、阿魏酸含量最高。结论该方法稳定、可行,可为该药对的进一步研究提供理论依据。

Objective To optimize the extraction procedure of the couple herbs,Radix paeoniae Alba and Rhizoma chuanxiong.Methods Orthogonal design was employed to optimize the extracting procedure.The content of three ingredients（gallic acid,paeoniflorin,ferulic acid） were used as index,the concentration of alcohol,the amount of alcohol,the extraction time,and the number of extraction times were used to study the effect on the content.Results The optimial preparation conditions were as follows：adding 12 times 50% alcohol,reflowing extraction 2 times,2 hours each time.Conclusion The established method was stable and feasible,and thus could provide a theoretical basis for the further research of the couple herbs.