大豆铁蛋白(Ferritin)基因家族的克隆与表达

Cloning and expression analysis of soybean ferritin gene family

从大豆中克隆到铁蛋白基因家族的13个成员,分别命名为GmFer1;1、GmFer2;1、GmFer2;2、GmFer3;1、GmFer3;2、GmFer7;1、GmFer10;1、GmFer11;1、GmFer14;1、GmFer14;2、GmFer18;1、GmFer18;2和GmFer18;3,它们之间序列相似性介于37.2%～99.1%,并且具有保守类Ferritin结构域,被聚为3个亚家族(GroupI、II和III)。大豆铁蛋白基因在根、茎、叶和种子中均表达,100μmol/L的柠檬酸铁处理后,大豆铁蛋白基因在根、茎和叶中的表达均受到强烈诱导,表达量显著增强。随着大豆种子的不断发育,大豆铁蛋白基因的表达量呈现出早期积累,后期下降的变化趋势。

Ferritin is an iron storage protein combined with iron regulating intracellular free iron ion concentration.There are two main roles of ferritin in vivo,one is to provide iron for intracellular iron protein;another to clear the iron-mediated free radical reactions.In this study,a total of 13 members of ferritin gene were cloned in soybean,they were GmFer1;1,GmFer2;1,GmFer2;2,GmFer3;1,GmFer3;2,GmFer7;1,GmFer10;1,GmFer11;1,GmFer14;1,GmFer14;2,GmFer18;1,GmFer18;2 and GmFer18;3.The sequence similarity of the soybean ferritin family members ranged from 46.6% to 93.1%,and had ferritin-like conserved domain.The soybean ferritin family were clustered into three subfamilies（GroupI,II and III）.Expression analysis showed that soybean ferritin genes were expressed in root,stem,leaf and seed.Soybean ferritin genes were strongly induced in root,stem and leaf.Their expression was significantly enhanced under 100μmol/L ferric citrate treatment.With the continuous development of soybean seeds,the expression of soybean ferritin genes showed early accumulation and late decline trend.