杜马斯燃烧法测定油料粗蛋白含量

Dumas combustion method for determination of crude protein content in oilseeds and products

通过优化杜马斯燃烧法测定油料粗蛋白含量条件,比较了杜马斯燃烧法和凯氏定氮法测定大豆、花生、油菜籽、芝麻和菜籽饼粕等油料样品粗蛋白含量。结果表明:杜马斯燃烧法与凯氏定氮法测定结果呈显著线性相关(线性相关系数为0.998 8,P<0.001),杜马斯燃烧法与凯氏定氮法在测定样品粗蛋白时无显著性差异(P>0.05),并且燃烧法变异系数CV小于0.6%,测定结果准确、重现性好,更适用于油料样品粗蛋白含量检测。

Dumas combustion method for determination of crude protein content in oilseeds and products was optimized in this study.The crude protein contents in samples of soybean,peanut,rapeseed,sesame and rapeseed meal were comparatively tested with both Dumas combustion and Kjeldahl method.Detected values from Dumas combustion method showed a significant linear correlation（r=0.998 8,P0.001） with Kjeldahl method and the results of Dumas method were more stable.Crude protein results in samples by both methods had no significant differentce（P0.05） and the coefficient of variation of Dumas combustion was lower than Kjeldahl（CV0.6%）.The results indicated that Dumas combustion method could be applied in the analysis of crude protein contents in oilseed samples.