抗真菌单体组分DZP-8和DZP-9理化性质及其稳定性研究

Studies on Stability of Physical and Chemical Properties of Antifungal Monomers DZP-8 and DZP-9 Produced by Streptomyces 702

从链霉菌702发酵液中分离得到DZP-8和DZP-9两种抗真菌活性物质单体组分,分别对其进行了理化性质及其稳定性研究。研究结果表明:DZP-8为淡黄色粉末状物质,熔点170～173.5℃;旋光度为[α]23.7D=+4.342 5°(c=1.09 in MeOH),紫外光谱表明在318,303,290 nm处有3个典型吸收峰,符合多烯类抗生素图谱特征,红外光谱表明其含有C=O、C=C、C-O、OH和CH2。化合物DZP-9,黄色无定形粉末,熔点205～207℃,[α]-177D(in MeOH);紫外光谱显示在337、340和320 nm处有最大吸收,表明其结构中存在较大共轭体系。红外光谱显示DZP-9分子中可能含有羟基(3 417、1 066、1 006 cm-1),酯键(1 723、1 172、1 137 cm-1),共轭双键(3 023、1 639、849 cm-1)。稳定性试验结果表明:DZP-8和DZP-9对热较稳定,温度超过60℃活性开始下降;在强酸强碱条件下活性不稳定,pH=5～6时活性稳定;短时间内对紫外线照射和光照具有较好的稳定性。本研究为链霉菌702所产抗真菌单体组分的开发应用提供了有益的试验数据。

The physical and chemical properties and stability of antifungal component DZP-8 and DZP-9 isolated from Streptomyces 702 fermentation were determined.The results of the study on the physical and chemical properties showed that DZP-8 was light yellow amorphous powder,its melting point was 170-173.5 ℃,and its rotation was [α]23.7D=＋4.342 5°（c =1.09 in MeOH）.UV spectrum showed that DZP-8 had three peaks at 318 nm,303 nm,290 nm and was in accordance with the characters of tatar-alkene antibiotic.IR spectrum showed that DZP-8 contained C=O,C=C,C-O,OH and CH 2.DZP-9 was yellow amorphous powder,and its melting point was 205-207 ℃,and its rotation was [α]-177 D（in MeOH）.UV spectrum showed that DZP-9 had maximum absorption at 337 nm,340 nm and 320 nm,and showed that its structure was a comparative large conjugate system.IR spectrum showed that DZP-9 may contained hydroxy（3 417,1 066,1 006 cm-1）,ester bond（1 723,1 172,1 137 cm-1）,and conjugated double bond（3 023,1 639,849 cm-1）.The results of the test on the stability showed that DZP-8 and DZP-9 were stable to thermal,UV and light in short time,but the activity reduced over 60 ℃.Its activity was stable when pH was 5 or 6,but unstable under strong acid and strong base.The studies provided meaningful test data for the application of antifungal monomers produced by Streptomyces 702.