KLF4对人胃癌细胞株SGC-7901体外增殖及迁移侵袭能力的影响

Effects of KLF4 on proliferation,migration and invasion of human gastric cancer SGC-7901 cells in vitro

目的探讨Krüppel样因子4(KLF4)对人胃癌SGC-7901细胞株增殖和迁移侵袭能力的影响。方法以人胃癌SGC-7901细胞株为研究对象,脂质体法转染pcDNA3.1IE-KLF4重组质粒并建立KLF4稳定过表达细胞株,以未转染KLF4基因和转染空质粒载体的胃癌细胞株为对照。逆转录聚合酶链反应(RT-PCR)检测KLF4mRNA的表达,四甲基偶氮唑盐(MTT)法检测转染后SGC-7901细胞的增殖情况,划痕试验和Transwell小室检测胃癌细胞迁移侵袭能力。结果与未转染组和空载体组比较,转染组细胞KLF4mRNA表达明显,MTT法检测显示48h和72h细胞增殖抑制明显(P<0.05),24、48、72h的增殖抑制率分别为12.90%、23.85%、25.56%。未转染组、空载体组和转染组细胞迁移率分别为(78.15±4.86)%、(73.75±4.03)%、(60.84±5.56)%,侵袭穿膜细胞数分别为(163.53±13.95)个、(158.07±12.54)个、(73.87±8.70)个,转染组细胞迁移率和侵袭穿膜细胞数较对照组均降低(P<0.05)。结论 KLF4对胃癌SGC-7901细胞的体外增殖及迁移侵袭具有抑制作用。

Abstract：Objective To explore the effects of Krfippel-like factor 4（KLF4） on proliferation,migration and invasion of human gastric carcinoma cell line SGC-7901 in vitro. Methods Human gastric cancer SGC-7901 cells were subjected to liposome-mediated transfection with recombinant plasmid pcDNA3. IIE-KLF4 to establish a recombinant eel1 line for stable over-expression of KLF4. Meanwhile,non-transfected SGC-7901 cells and SGC-7901 cells transfected with empty plasmid vector were used as controls. The expression of KLF4 mRNA in each group was examined by reverse transcriptase-polymerase chain reaction（RT-PCR）. Proliferations of gastric carcinoma cells after transfection were examined by methyl thiazolyl tetrazolium（MTT） assay. Migration and inva sion of gastric carcinoma cells were examined by wound healing assay and Transwell chamber assay,respectively. Results The expression of KLF4 mRNA was significant in cells transfected with KLF4 as compared with controls. MTT assay results showed that proliferation of cells transfected with KLF4 was significantly inhibited at 48 h and 72 h（P〈0.05）. Inhibition rates at 24,48 and 72 h were 12.90% ,23.85% and 25.56% ,respectively. Migration rates of each group were （78.15±4.86） % , （73. 75±4.03） % and （60.84±5.56） % ,respectively. Numbers of cells crossed the membrane of each group were 163.53±13.95,158. 07± 12.54 and 73.87 ± 8.70, respectively. Migration rate and number of cells crossed the membrane in group transfected with KLF4 were both decreased in contrast with controls （P〈0.05）. Conclusion KLF4 could inhibit the proliferation,migration and invasion of SGC 7901 cell line in vitro.