慢性激光刺激对人体衰老相关指标的影响

Effects of chronic laser stimulation on human aging-related indicators

目的了解慢性激光刺激对人体衰老相关指标的影响。方法将168名某激光研究所人员按是否进行激光作业分为暴露组(n=90)和非暴露组(n=78)。采用固相夹心法酶联免疫吸附测定(ELISA)检测血浆Klotho蛋白含量,采用比色法检测红细胞超氧化物歧化酶(SOD)活力及丙二醛(MDA)浓度,采用流式荧光原位杂交(Flow-FISH)检测淋巴细胞端粒长度。结果暴露组及非暴露组人员血浆Klotho蛋白水平、红细胞MDA浓度、淋巴细胞端粒长度的差异无统计学意义(P>0.05);多元回归分析显示血浆Klotho蛋白、红细胞SOD与MDA、淋巴细胞端粒长度与激光暴露无明显相关,亚组分析显示暴露组中吸烟人员的红细胞SOD浓度明显低于非暴露组(P<0.05)。结论慢性激光刺激对衰老相关指标无直接影响,但可能导致吸烟人员红细胞SOD浓度降低。

Objective To understand effects of chronic laser stimulation on human aging-related indicators. Methods 168 staffs in a certain laser institute were divided into exposed group（n=90） and non-exposed group（n=78） according to whether or not participating laser job. Solid-phase sandwich enzyme-linked immunosorbent assay（ELISA） was used to detect plasma Klotho protein content, colorimetrie detection was employed to measure erythrocyte superoxide dismutase （SOD） and malondialdehyde（MDA） concentration, and flow fluorescence in situ hybridization （Flow-FISH） was adopted to test length of telomere in lymphocyte. Results Between exposed group and non exposed group, differences of plasma Klotho protein level, erythrocyte MDA concentration and lymphocyte telomere lengths were not statistically significant（P〉0.05）. According to multiple regression analysis, plasma Klotho protein, erythrocyte SOD and MDA,lymphocyte telomere length showed no correlation with laser exposure. Subgroup analysis demonstrated that concentrations of erythrocyte SOD of smoking staffs in exposed group were markedly lower than those in non-exposed group（P〈0.05）. Conclusion Chronic laser stimulation has no direct effects on aging-related indicators,but may lead to decreasing erythrocyte SOD concentration in smoking person.