胃癌组织和胃癌细胞株中Claudin-6、CD44V6的表达及其临床意义

Expression and clinical significance of Claudin-6 and CD44V6 expression in gastric carcinoma tissues and cell lines

目的研究胃癌组织和胃癌细胞中Claudin-6、CD44V6蛋白的表达及其临床意义。方法应用免疫组织化学及免疫细胞化学法检测Claudin-6及CD44V6蛋白在正常胃黏膜、不典型增生胃黏膜、胃腺癌组织及胃癌MKN28、SGC7901、BGC823细胞中的表达。结果正常胃黏膜、不典型增生胃黏膜及胃腺癌组织中,Claudin-6的阳性表达率分别为100.0%、65.0%、53.3%,呈明显降低趋势(χ2=7.758,P<0.05);CD44V6的阳性表达率分别为20.0%、45.0%、77.8%,呈明显增高趋势(χ2=15.917,P<0.05)。肿瘤侵及浆膜层者Claudin-6蛋白的阳性表达率明显低于侵及肌层及以内者(P<0.05);而其CD44V6蛋白的阳性表达率明显高于侵及肌层及以内者(P<0.05)。有淋巴结转移者Claudin-6蛋白的阳性表达率明显低于无淋巴结转移者(P<0.05),而其CD44V6蛋白的阳性表达率明显高于无淋巴结转移者(P<0.05)。在不同性别、年龄、分化程度及有无脉管侵犯的患者中,Claudin-6和CD44V6蛋白阳性表达率的差异均无统计学意义(P>0.05)。Spearman等级相关性分析显示,Claudin-6与CD44V6表达呈负相关(r=-0.986,P<0.05)。在MKN28、SGC7901、BGC823细胞中,Claudin-6和CD44V6免疫染色强度的差异无统计学意义(P>0.05)。结论 Claudin-6及CD44V6在胃癌发生、发展中有一定作用,检测Claudin-6及CD44V6的表达有助于判断胃癌患者的病情和预后。

Objective To study the expression and clinical significance of Claudin-6 and CD44V6 protein expression in gastric carcinoma tissues and cell lines. Methods Immunohistochemistry and immunocytochemistry were applied to assay the expression of Claudin-6 and CD44V6 protein in normal gastric mucosa, gastric mucosa with atypical hyperplasia, gastric adenocareinoma tissues and gastric cancer MKN28,SGC7901 ,BGC823 ceils. Results The positive expression rates of Claudin-6 in normal gastric mucosa, gastric mucosa with atypical hyperplasia and gastric adenocarcinoma tissues were 100.0 %, 65.0 % and 53. 3 %, respectively, with significantly decreasing trend（χ^2= 7. 758, P〈0.05）, and the positive expression rates of CD44V6 in tissues above were 20.0%, 45.0 % and 77.8 %, respectively, with obviously increasing trend（ χ^2= 15. 917, P〈0.05）. In tissues with tumor invading serosa or lymph node metastasis,the positive expression rates of Claudin 6 protein were significantly lower than that invading less than muscle or without metastasis（P〈0.05）, while those of CD44V6 protein were markedly higher（P〈 0.05）. Among different gender, age,differentiation degree and whether or not vascular invasion, differences of positive expression rates of Claudin-6 and CD44V6 were not statistically significant（P〉0.05）. Spearman rank correlation analysis showed that Claudin 6 and CD44V6 expression was negatively correlated（r=0. 986 ,P〈0.05）. Differences of immunostaining intensities of Claudin-6 and CD44V6 among MKN28, SGC7901 ,BGC823 cells were not statistically significant（P〉0.05）. Conclusion Claudin-6 and CD44V6 play a certain role in carcinogenesis and progression of gastric carcinoma,and detection of Claudin-6 and CD44V6 expression may be useful in estimating the condition and prognosis of patients with gastric carcinoma.