猪伪狂犬病病毒桂W株的分离鉴定

Isolation and Identification of GW strain of Pseudorabies Virus from piglet

从南宁市郊某猪场采集的发病仔猪大脑和内脏病料中分离到一株病毒。病毒接种家兔后引起典型的伪狂犬病症状而死。病毒接种RK细胞出现典型的细胞病变 ,感染细胞形成合胞体。毒价 (TCID50 )为 10 7.86/0 .1ml。病毒能被伪狂犬病病毒标准阳性血清中和 ,血清中和效价为 1∶10 7。电镜观察到圆形、有囊膜、直径 80～ 12 0nm大小的病毒颗粒。PCR反应可扩增特异性 2 17bpDNA片段。证实分离的病毒株为伪狂犬病病毒 ,并命名为伪狂犬病病毒桂W株。

One virus was isolated from brain and visceral organs of diseased piglets from a pig farm in Nanning.The virus cause infected rabbits typical pseudorabies clinical synptom and death.The virus induced typical cytopathogenic effect(CPE)after inoculated in rabbit kidney(RK)cell,inffected RK cell .formed syncytium.Virus TCID 50 was 10 7.86 /0.1ml.The virus could be neutralized by standard positive serum of pseudorabies virus,serum neutralization titre was1∶107.Round,with envelope,80～120nm diameter virus particles could be found under electromicroscope.A specific 217 bp long DNA fragment could be expanded using polymerase chain reaction(PCR) analysis from the isolated virus DNA.It was indicated that the isolated virus was pseudorabies virus (PRV),it was named pseudorabies virus GW strain.