摘要
以伪狂犬病病毒Fa株为起始材料 ,构建的 pp6 3LacZ转移载体质粒与PRVFaDNA经磷酸钙转染 ,在MDBK细胞内同源重组获得PRVFagE-/gI-/LacZ基因缺失株。以PDTK3 - 6 ,5 - 6TK缺失质粒与上述基因缺失株在TK-143细胞内同源重组 ,获得PRV -SA2 15等三基因缺失株。经核酸杂交证实构建是成功的。生物学特性观察显示 ,该基因缺失株对多种动物安全 ,具有良好的免疫原性 ,有望成为一株优秀的PRV疫苗候选株。
By cotransfection of PRV Fa DNA and the shuttle vector plasmid pp63LacZ DNA in MDBK cells, a deletion mutant strain PRV Fa gE-/gI-/LacZ was constructed with calcium phosphate transfection system. By cotransfection of PRV Fa gE-/gI-/LacZ DNA and TK gene deleted plasmid PDTK3-6, 5-6 DNA, recombinant viruses were then selected for 5'-BUDR resistance in TK-143 cells and Assayed by using the methods of Dot-Blot hybridization, the results suggest PRV Fa TK-/gE-/gI-/LacZ is constructed successfully, the study of biologic characteristics show that vaccination various animals with the gene deleted strain is safe and effective.
出处
《四川农业大学学报》
CSCD
2000年第1期1-3,10,共4页
Journal of Sichuan Agricultural University
基金
国家自然科学基金! (批准号 :395 70 5 44 )
国家"九五"攻关项目资助。
关键词
伪狂犬病病毒
基因缺失
疫苗
生物学特性
PSEUDORABIES VIRUS
GENE DELETED
VACCINE
BIOLOGIC CHARACTERISTICS
