几种特异小麦的醇溶蛋白电泳分析

Gliadin Analysis of Some Peculiar Wheats

应用酸性聚丙烯酰胺凝胶电泳 (APAGE)对 4份波兰小麦、2份新疆稻麦、1份硬粒小麦和 1份矮兰麦进行了醇溶蛋白电泳分析 ,结果表明 :①波兰小麦具有明显的醇溶蛋白多态性 (多样性 ) ,其中的矮秆波兰小麦的谱带与产于新疆吐鲁番的波兰小麦极为相似 ;②波兰小麦与硬粒小麦、矮兰麦的α -醇溶蛋白和ω -醇溶蛋白的区别较明显。硬粒小麦和矮兰麦在α区域的带纹明显较波兰小麦多 ;③波兰小麦与新疆稻麦的醇溶蛋白电泳图谱具有一定的相似性 ,产于新疆和田的波兰小麦与新疆稻麦的共同醇溶蛋白带纹达到 6 2 5 % ,表明新疆稻麦与波兰小麦具有较近的亲缘关系 ,从醇溶蛋白方面证明了新疆稻麦起源于波兰小麦的可能性。

The gliadin patterns of four Triticum polonicum, two T.Petropavlovskyi, one T.durum and one T.turgidum were analyzed by using acid polyacrylamide gel electrophoresis (APAGE). The results are as follows: ①T.polonicum has significant gliadin polymorphism, the patterns of dwarfing T.polonicum are similar to those of the T.polonicum in Tulufan, Xinjiang. ②The α-gliadin and ω-gliadin of T.polonicum are significantly different from those of T.durum and T.turgidum, with more α-gliadin patterns than the former. ③With 62.5% of common gliadin patterns, the gliadin patterns of T.polonicum in Hetian, Xijiang and those of T.petropavlovskyi have much in common, which indicates that T.petropavlovskyi and T.polonicum have close affinity, thus proving that T.petropavlovskyi most probably originates from T.polonicum in terms of gliadin.