中压制备系统联合自动纯化系统制备淫羊藿中的朝藿定A、B、C对照品

Preparation of Epimedins A,B,C from Epimedium brevicornum Maxim Using Middle Pressure Preparative System Combined Autopurification System

目的采用中压制备系统联合自动纯化系统从淫羊藿原药材中分离制备朝藿定A、B、C 3种成分。方法淫羊藿提取物经大孔吸附树脂粗分离获得相应的组分后,利用中压制备系统联合自动纯化系统完成精制纯化。结果从4.5kg淫羊藿原药材(总黄酮含量约5%)中获得朝藿定A 2.4g、朝藿定B 14.8g和朝藿定C 1.7g,纯度均达到98%以上。结论此方法通过三步分离即可实现朝藿定A、B、C 3种成分的完全分离,具有高效快速、产品纯度高的特点,适于淫羊藿中朝藿定A、B、C系列对照品的规模制备。

OBJECTIVE Middle pressure preparative system combined autopurification system was developed to purity Epi- medins A, B, C as reference standards from the crude extract of Epimedium brevicornum Maxim. METHODS After the crude extract containing 5% flavonols was enriched using macropore resin, the obtained target fractions were subjected to middle pressure preparative system and then to autopurification system. RESULTS By repetitive injection of the enriched target frac- tion on to the preparative column, 2.4 g epimedin A as well as 14.8 g epimedin B and 1.7 g epimedin C were obtained from 4. 5 kg Epimedium brevicornum Maxim raw herbs. The purities of all products were greater than 98%. CONCLUSION The middle pressure preparative system combined autopurification system technique for three target compounds are quite useful for the production of the reference standard series with good putity like epimedins A, B, C standards due to its rapid separation, high performance and more amounts of products obtained.